Enzyme Concentration On The Rate Of Reaction

INTRODUCTION: I am trying to investigate enzyme extinguish and the rate of reaction. In this investigation, a proteolytic enzyme is used to transform state the breakdown of protein into peptides. Protein colloidal gel is the substrate used. As the reaction progress, the gelatine is broken down and become slight viscous. This has effect on the time it takes for the gelatin to go finished the move in to the beaker. HYPOTHESIS: I predicted the higher the enzyme submergence, the less(prenominal) time it volition take for the gelatin to pass through the funnel because the more than the proteolytic enzyme concentration, the faster the breakdown of the protein (gelatin) into peptides. EQUIPMENTS/ machine: Pipette, try pipage, trypsin, gelatin (protein), beaker, glass rod, clamp stand, funnel, discover time and ph buffer. subprogram: I used the pipette to total 3 cm of pH buffer into a try out tube. The test tube was handled with c be to prevent it from breaking. I then used some other pipette to heartbeat 7cm of 0% trypsin firmness into the same test tube containing the pH buffer. 75 gram of gelatin was weighed into a beaker. I then peppered the mixture of pH buffer and the trypsin solution in the beaker containing 75 gram of gelatine. Using the glass rod, I carefully and thinly stir the mixture for approximately 15 seconds, using the stop clock.

Using a clamp stand, I suspended the funnel over an empty beaker. I pour whole the mixture into the funnel after leaving it for 5 minutes and again started the stop clock. I recorded all the time interpreted for th e gelatin to pass through the funnel. I r! epeated the role for 1%, 3% and 5% of enzyme concentration inconstantS: IN babelike VARIABLE: Enzyme concentration (trypsin) was the main(a) variable because they are the changes that were directly caused by me. DEPENDENT VARIABLE: Rate of reaction (1/T) that is the time taken for the gelatine to pass through the funnel. This was the independent variable because it was the changes...If you want to arouse a full essay, enunciate it on our website: OrderCustomPaper.com

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